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Lymphocytes circulating in the blood are normally spherical and covered with microvilli. Activated to migrate by chemokine, a lymphocyte polarizes its cytoskeleton and organelles as it retracts its microvilli. An array of microtubules and 10nm vimentin filaments create cell rigidity that stabilizes shape or inhibits cell movement. Ratner proposes, that removal of microtubules to the uropod releases cells from inhibition. Betsy Repasky finds that Protein kinase C and Spectrin move from the cell periphery to the uropod; and, the Shaw Lab finds that vimentin, plectin and fodrin also go to the uropod quickly in response to chemokine signals directing cell polarity and movement. Cytoskeletal polarization seems to be aligned so that acquisition of signal and start of movement are correctly at opposite ends of the cell. Nieto et al saw initial expression of chemokine receptors is at the leading edge. Adhesion receptors are first expressed there also, but move to the uropod upon migration.
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